Relationship between membrane and cytoplasmic domains in cytochrome c oxidase by electron microscopy in media of different density |
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Authors: | J F Deatherage R Henderson R A Capaldi |
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Institution: | Medical Research Council Laboratory of Molecular Biology Hills Road, Cambridge, England;Institute of Molecular Biology University of Oregon, Eugene, Ore, 97403, U.S.A. |
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Abstract: | We have investigated the relative location of the exposed cytoplasmic and membrane domains in cytoehrome c oxidase vesicle crystals by varying the density of the embedding medium in electron microscopy. This gives the connectivity of the domains, revealing a Y-shaped cytoehrome c oxidase monomer. A large domain, the stem of the Y, projects over 50 Å into solution from the side of the crystal membrane corresponding to the cytoplasmic face of the inner mitochondrial membrane. Two smaller domains are embedded in the bilayer and must be largely separated by lipid. The Y-shaped cytochrome c oxidase monomers are compactly paired as dimers. |
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Keywords: | Author to whom all correspondence should be addressed |
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