Excystation and development in the chick and on the chick chorioallantois of the metacercaria of Sphaeridiotrema globulus (Trematoda) |
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Authors: | Bernard Fried Jane E Huffman |
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Institution: | 1. Department of Biology, Lafayette College, Easton, PA 18042, U.S.A.;2. Department of Zoology and Physiology, Parasitology Laboratory, Rutgers The State University, Newark, NJ 07102, U.S.A. |
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Abstract: | Fried B. and Huffman J. E. 1982. Excystation and development in the chick and on the chick chorioallantois of the metacercaria of Sphaeridiotrema globulus (Trematoda). International Journal for Parasitology12: 427–431. Encysted metacercariae of Sphaeridiotrema globulus were obtained from naturally infected Goniobasis virginica snails in Morris County, New Jersey, U.S.A. These metacercariae were successfully excysted within l h in an alkaline bile salts trypsin medium maintained at 41°C in the absence of acid pepsin pretreatment. Encysted metacercariae treated in 3% sodium bicarbonate produced successful infections in day-old domestic chicks and worms became ovigerous in the lower ileum by day 4. Excysted metacercariae transferred to the chorioallantois of 6–10-day-old chick embryos maintained at 41°C developed into ovigerous adults by day 4. |
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Keywords: | Trematoda chemical excystation chorioallantois domestic chick cultivation encysted metacercariae excysted metacercariae development |
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