水稻苗期耐旱性基因位点及其互作的分析

随着全球水资源的日益贫乏和旱灾的日趋严重，水稻耐旱性的研究越来越重要，对籼稻窄叶青8号（ZYQ8）和粳稻京系17（JX17）以及由它们构建的加倍单倍体（DH）群体，参照国际水稻研究所的耐旱鉴定方法，在苗期进行断水，调查期耐旱性，利用该群体的分子连锁图谱进行数量性状座位（QTL）区间作图分析，共检测到2个耐旱的QTL（qDT-5和qDT-12)，分别位于第5染色体的GA41-GA257之间的和第12染色体的RG457-Y12817R之间，这两个QTL的加性效应均来自ZYQ8的等位基因，用Epistat软件检测到2个单位点，即GA257和Y12817R，与区间作图分析的结果一致，Epistat还检测到与GA257互作的3个位点（RG541、G318和G192，分别位于第1、4和8染色体上）和与Y12817R互作的1个位点（CT234，位于第3染色体上）。

Analysis of Gene Loci and Epistasis for Drought Tolerance in Seedling Stage of Rice (OryZa sativa L.)

Drought tolerance of rice is important because a considerable proportion of the world rice area is not irrigated and is prone to water deficit. In this study, an indica variety, Zhai Ye Qing 8 (ZYQ8), and a japonica variety, Jing Xi 17 (JX17), and their double haploid (DH) population were used for genetic study of drought tolerance. Water supply was stopped in seedling period for 15 days and then drought tolerance of the DH population and their parents were investigated. Mapping quantitative trait loci (QTLs) was undertaken base on the constructed molecular linkage map of this population. Two QTLs ( qDR 5 and qDR 12) for drought tolerance were identified, they were in the region of GA41 GA257 on chromosome 5 and RG457 Y12817R on chromosome 12, respectively. The tolerance alleles of both QTLs were from the indica parent, ZYQ8. In the meantime two genes for drought tolerance near GA257 and Y12817R were detected too by usingEpistat software, that is in accordance with the result by using Mapmaker/QTL. In addition, three loci ( RG541, G318 and G 192 on chromosome 1,4 and 8, respectively) were found interacting with GA257 by Epistat software, while one locus ( CT 234 on chromosome 3) found interacting with Y12817R were also detected by Epistat software.