Identification of mycotoxins in keratomycosis-derived Fusarium isolates by gas chromatography—mass spectrometry |
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Authors: | SK Raza SA Howell AI Mallet |
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Institution: | St John's Institute of Dermatology, UMDS, University of London, St. Thomas' Hospital, London SE1 7EH UK |
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Abstract: | The production of mycotoxins from Fusarium species has been demonstrated in isolates cultured from patients suffering from keratomycosis. The method employed a combination of thin-layer chromatography directly performed on gel plugs taken from the growth medium, cartridge column chromatography, silylation and gas chromatography on a non-polar stationary phase capillary column linked to mass spectrometry. The sensitivities of detection obtained for a signal-to-noise ratio of 33:1, were 200 pg for single stage GC-MS and 20 pg using tandem GC-MS-MS. Two mycotoxins, diacetoxyscirpenol and T-2 toxin were identified in three cultures. |
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