天然药物对牙龈卟啉单胞菌的体外抗菌研究

目的 观察天然药物对牙龈卟啉单胞菌（Porphyromonas gingivalis,P.gingivalis）生长的影响,筛选抗菌作用最强的天然药物。方法 选用7种天然药物,采用液体稀释法,结合吸光度测定以确定7种天然药物对P.gingivalis的最小抑菌浓度（MIC）与最小杀菌浓度（MBC）。结果 黄芩、三七、白芷、大黄、五倍子、血藤和川芎对P.gingivalis的MIC值分别为1、1、0.5、0.0156、1、0.5、0.125 mg/ml;MBC值分别为1、1、0.5、0.0625、1、1、0.25mg/ml。结论 7种天然药物对P.gingivalis的生长均表现出明显的抑制作用,大黄的抑菌作用最强,川芎其次。

Antimicrobial effect of natural medicines on Porphyromonas gingivalis in vitro

Objective To investigate the antimicrobial effect of seven natural medicines on Porphyromonas gingivalis.Methods The liquid media two-fold dilution method and determination of A was performed to determine minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of each medicine.Results MIC for Radix Scutellariae,Radix Notoginseng,Radix Angelicae Dahhuricae,Radix et Rhizoma Rhei,Galla Chinensis,Caulis Sargentodoxae,Rhizoma Chuanxiong were 1 mg/ml,1 mg/ml,0.5 mg/ml,0.0156 mg/ml,(1 mg/ml),0.5 mg/ml and 0.125 mg/ml,respectively.MBC for Radix Scutellariae,Radix Notoginseng,Radix Angelicae Dahuricae,Radix et Rhizoma Rhei,Galla Chinensis,Caulis Sargentodoxae,Rhizoma Chuanxiong were 1 mg/ml,1 mg/ml,0.5 mg/ml,0.0625 mg/ml,1 mg/ml,1 mg/ml and 0.25 mg/ml,respectively.Conclusion Extract of the seven natural medicines showed an effective antimicrobial activity against Porphyromonas gingivalis.Among them,Radix et Rhizoma Rhei inhibited growth of Porphyromonas gingivalis most effectively.