Assessment of vegetative compatibility of race-2 tomato wilt isolates ofVerticillium dahliae in Japan |
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| Authors: | Hideyuki Nagao Toshimasa Shiraishi Satoko Oshima Masanori Koike Tsutomu Iijima |
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| Institution: | (1) Faculty of Horticulture, Chiba University, 271 Matsudo, Japan;(2) Gunma Horticultural Experiment Station, 379-22 Gunma, Japan;(3) Sakata Seed Co. Ltd., 358, Uchikoshi, 299-02 Sodegaura, Chiba, Japan;(4) Verticillium Research Group, Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, 080 Obihiro, Japan;(5) Tokyo Metropolitan Agricultural Experiment Station, 180 Tokyo, Japan |
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| Abstract: | Verticillium dahliae race-2 can invade the resistant cultivars of tomato possessing theVe gene. This new race was recently found in several regions in Japan, and 10 isolates ofV. dahliae race-2 from these regions were used in our study. Pathogenicity tests identified these isolates as the tomato pathotype (B).
We examined the vegetative compatibility of 8 of these 10 Japanese isolates ofV. dahliae race-2 to estimate their genetic relatedness with the testers of Japanese vegetative compatibility group previously proposed
(VCGJ) usingnit mutants. Compatiblenit1 and NitM mutants were obtained from allV. dahliae race-2 isolates. Selected representativenit1 and NitM mutants of eachV. dahliae race-2 isolates were paired with VCGJ testers. All isolates ofV. dahliae race-2 showed a strong reaction with VCGJ2, i.e., tomato pathotype. All isolates ofV. dahliae race-2 except for isolate To22 reacted weakly to VCGJ1 and J3. Japanese isolates ofV. dahliae race-2 were assigned as VCGJ2 and were hence vegetatively closely related with those ofV. dahliae race-1. The origin of Japanese isolates ofV. dahliae race-2 was discussed. |
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| Keywords: | nit tomato VCG Ve gene Verticillium dahliae race-2 |
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