Detection and pathological role of intestinal protozoa in children |
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| Institution: | 1. Unidad de Microbiología, Hospital Universitari Arnau de Vilanova, Lleida. Spain;2. Universitat de Lleida, Lleida, Catalunya, Spain;1. Grupo de Investigaciones Microbiológicas, UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá, Colombia;2. Grupo de Parasitología, Instituto Nacional de Salud, Bogotá, Colombia;3. Programa Profesional de Biología, Grupo de Investigación en Parasitología y Microbiología Tropical, GIPAMT, Universidad Incca de Colombia, Bogotá, Colombia;4. Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia;5. Instituto de Investigaciones Biomédicas, Facultad de Medicina, Universidad Mayor de San Simón, Cochabamba, Bolivia;6. Laboratorio de Investigaciones FEPIS, Quininde, Esmeraldas Province, Ecuador;7. Facultad de Ciencias Médicas, de la Salud y la Vida, Universidad Internacional del Ecuador, Quito, Ecuador;8. Departamento de Parasitología del Hospital Nacional de Clínicas, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina;1. Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan;2. Department of Parasitology, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan;3. Asian Health Science Research Institute, Osaka Prefecture University, Osaka 598-8531, Japan;4. Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia;5. Chiyoda Kohan Co., Ltd., Tokyo 104-8115, Japan;6. Department of Biomedical Engineering, Faculty of Engineering, Okayama University of Science, Okayama 700-0005, Japan;7. Department of Parasitology and Research Centre for Infectious Disease Sciences, Graduate School of Medicine, Osaka City University, Osaka 545-8585, Japan;1. Faculty of Earth and Life Sciences, VU Amsterdam University, De Boelelaan 1105, 1081, HV, Amsterdam, the Netherlands;2. Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro. Avenida de las Ciencias s/n, Juriquilla 76230, Queretaro, Mexico;3. Department of Biomedical Sciences, Unit of Medical Helminthology, Institute of Tropical Medicine. Kronenburgstraat 43, 2000 Antwerpen, Belgium;1. Statens Serum Institut, Copenhagen, Denmark;2. National University of Singapore, Singapore;3. London School of Hygiene and Tropical Medicine, London, UK;1. Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain;2. Clinical Research Unit, IMAS12-CIBERESP, Hospital Universitario 12 de Octubre, Madrid, Spain |
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| Abstract: | IntroductionIntestinal parasites are considered a growing public health problem, being protozoa the main cause of intestinal disease. The objective of our study is to compare the detection of intestinal protozoa by microscopy versus real-time PCR, as well as to determine the most prevalent protozoa in our environment in the paediatric population.MethodAn observational longitudinal study was carried out, both by microscopy and real time-PCR in stool samples from children (0‐ 15 years) received from April 2019 to March 2021.Children were classified in two groups according if they had or not had clinical parasitosis.Microscopic examination was performed in all samples using the Ritchie concentration technique with the commercial Mini PARASEP system (Movaco-Grifols®). The presence of Cryptosporidium sp. was evaluated with the modified Ziehl-Neelsen acid-fast stain. The real-time PCR was performed to all samples using the Allplex ? gastrointestinal parasite panel 4 (Seegene®).ResultsDuring the study period, 500 samples were received, being positive 31 (6.2%) by microscopy and 256 (51.2 %) by PCR. By microscopy, Blastocystis hominis was the most frequently observed (4.8%), followed by Giardia lamblia (1.6%), Dientamoeba fragilis (0.2%) and Cryptosporidium species (0.2%). Regarding the identification by PCR, D. fragilis (35.2%) was mainly identified, followed by B. hominis (28.1%), G. lamblia (7%) and Cryptosporidium sp. (0.8%) without finding clear differences in aetiology according to age. In the case of B. hominis and D. fragilis, there were not differences in the detection of these protozoa between the control group and children with clinical parasitosis (p = 0.11).ConclusionsReal-time PCR increases the detection of intestinal protozoa, being underdiagnosed by microscopy, especially D. fragilis, in which PCR is considered the most appropriate method for its detection. |
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