Sensitivity of ATPase activities to fire ant venom and abdomen preparations. |
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Authors: | R B Koch D Desaiah |
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Institution: | Department of Biochemistry, Mississippi State University Mississippi State, Mississippi 39762 U.S.A. |
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Abstract: | The sensitivity of fire ant, (Forel), head homogenate ATPase to its venom and to a cyclohexane extract of whole fire ants were investigated. Na+K+ and oligomycin-sensitive Mg2+ ATPase activities were inhibited by both preparations. Oligomycin-insensitive Mg2+ ATPase activity was inhibited by low concentrations but showed strong stimulation at high concentrations of the venom preparations. Lineweaver-Burk plots of enzyme data in the presence or absence of inhibitor indicated that the inhibitor action was non-competitive with ATP for Na+K+ and oligomycin-sensitive Mg2+ ATPase activities. However, the oligomycin-insensitive Mg2+ ATPase activity showed a mixed type response to the inhibitor. Tests on pure samples of known venom components indicate that they cause the observed effects on the ATPase activities. |
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