罗丹明类荧光分子探针与血清白蛋白之间相互作用的研究

本研究旨在对罗丹明类荧光探针ZM-6与人血清白蛋白(HSA)的相互作用进行研究。采用了荧光光谱法、三维荧光光谱法、同步荧光光谱法以及CD光谱法在模拟生理条件下对二者的相互作用以及HSA的构象进行了研究。研究结果表明,探针与ZM-6之间的猝灭机理主要是静态猝灭方式。根据热力学数据确定了二者之间的作用力,类型为范德华力和氢键。二者之间的结合距离为4.45 nm。同时得出,ZM-6对HSA的构象产生了影响。此研究对于探针分子的设计以及修饰提供有效的数据以及理论支持。

Study on the Interaction between a Rhodamine-based Fluorescent Probe and Serum Albumin

This study aimed to study the interaction between a Rhodiamin-based fluorescent probe ZM-6 and human serum albumin(HSA). The interaction between the two and the conformation of HSA were investigated by fluorescence spectrum, the three dimensional fluorescence spectrum, synchronous fluorescence spectrum and CD spectrum methods under simulated physiological conditions. The results revealed that the quenching mechanism between probe ZM-6 and HSA was mainly on static quenching. The thermodynamic parameters revealed that the binding of(ZM-6)-HSA was relied on Van der Waals force and hydrogen bond. The binding distance of ZM-6 with HSA was calculated to be 4.45 nm. The results demonstrated that the conformation of HSA was affected by ZM-6. This study could provide effective data and theoretical support for the design and modification of probe molecules.