Generation and characterization of human monoclonal antibodies to G5, a linear neutralization epitope on glycoprotein of rabies virus, by phage display technology |
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Authors: | Zhao Xiao-Ling Yin Jun Chen Wei-Qiang Jiang Ming Yang Guan Yang Zhi-Hua |
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Institution: | Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin, China. xlzhao985@sohu.com |
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Abstract: | The aim of the present study was to discover distinct human MAbs to RV with high neutralizing potency and a broad neutralization spectrum. A phage display technology was used to produce human scFv to G5, a conserved linear neutralization epitope on Gp of RV. A phage display scFv library with 6 x 10(7) members was constructed and the phage-scFv with 'antigen-binding' activities were selected with synthetic peptide G5-24. The obtained scFv genes were cloned into pET22b(+)/BL21(DE3) and from this we prepared purified scFv fragments. The assay of the specificity characteristics and neutralization capacity showed that two distinct clones with new human immunoglobulin V genes can recognize G5 specifically as well as neutralize different RV strains. They have potential for inclusion in an antibodies combination aimed for use in rabies PEP. |
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Keywords: | G5 phage display rabies virus single-chain fragments |
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