Effects of acclimation salinity on the expression of selenoproteins in the tilapia,Oreochromis mossambicus |
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| Affiliation: | 1. Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813, USA;2. Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA;1. Departamento de Ciências da Nutrição, Universidade Federal da Bahia, Av. Araújo Pinho, 32, Canela, CEP: 40110-150 Salvador, Bahia, Brazil;2. Instituto de Matemática, Universidade Federal da Bahia, Av. Adhemar de Barros, s/n, Ondina, CEP: 40170-110 Salvador, Bahia, Brazil;3. Instituto de Saúde Coletiva, Universidade Federal da Bahia, Rua Basílio da Gama, s/n, Campus Universitário Canela, CEP: 40110-040 Salvador, Bahia, Brazil;4. Departamento de Ciências da Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, s/n, CEP: 40110-100 Salvador, Bahia, Brazil;1. Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA;2. Department of Population Health and Disease Prevention, University of California, Irvine, CA, USA;1. Centre for Biomolecular Interactions Bremen, Faculty 2 (Biology/Chemistry), University of Bremen, PO Box 330440, D-28334 Bremen, Germany;2. Centre for Environmental Research and Sustainable Technology, Leobener Strasse, D-28359 Bremen, Germany;3. Graduate School of Chemistry, University of Münster, Wilhelm-Klemm-Straße 10, D-48149 Münster, Germany;4. Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany;1. Institute of Molecular and Crystal Engineering, Henan Key Lab of Polyoxometalate Chemistry, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China;2. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China;3. Basic Experiment Teaching Center, Henan University, Kaifeng, Henan 475004, PR China |
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| Abstract: | Selenoproteins are ubiquitously expressed, act on a variety of physiological redox-related processes, and are mostly regulated by selenium levels in animals. To date, the expression of most selenoproteins has not been verified in euryhaline fish models. The Mozambique tilapia, Oreochromis mossambicus, a euryhaline cichlid fish, has a high tolerance for changes in salinity and survives in fresh water (FW) and seawater (SW) environments which differ greatly in selenium availability. In the present study, we searched EST databases for cichlid selenoprotein mRNAs and screened for their differential expression in FW and SW-acclimated tilapia. The expression of mRNAs encoding iodothyronine deiodinases 1, 2 and 3 (Dio1, Dio2, Dio3), Fep15, glutathione peroxidase 2, selenoproteins J, K, L, M, P, S, and W, was measured in the brain, eye, gill, kidney, liver, pituitary, muscle, and intraperitoneal white adipose tissue. Gene expression of selenophosphate synthetase 1, Secp43, and selenocysteine lyase, factors involved in selenoprotein synthesis or in selenium metabolism, were also measured. The highest variation in selenoprotein and synthesis factor mRNA expression between FW- and SW-acclimated fish was found in gill and kidney. While the branchial expression of Dio3 was increased upon transferring tilapia from SW to FW, the inverse effect was observed when fish were transferred from FW to SW. Protein content of Dio3 was higher in fish acclimated to FW than in those acclimated to SW. Together, these results outline tissue distribution of selenoproteins in FW and SW-acclimated tilapia, and indicate that at least Dio3 expression is regulated by environmental salinity. |
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| Keywords: | Fish Selenium Selenoprotein Acclimation salinity Tilapia Euryhaline |
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