| 含14-3-3蛋白抑制肽R18重组腺病毒制备及其在心肌细胞中的表达 |
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| 引用本文： | 廖文强,庞燕,于长安,李耿,温见燕,张翼冠,李晓辉.含14-3-3蛋白抑制肽R18重组腺病毒制备及其在心肌细胞中的表达[J].现代生物医学进展,2008,8(11). |
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| 作者姓名： | 廖文强 庞燕 于长安 李耿 温见燕 张翼冠 李晓辉 |
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| 作者单位： | 1. 中日友好医院全国中西医结合心血管病中心,北京,100029
2. 第三军医大学药学院,重庆,400038 |
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| 摘 要： | 目的:构建并鉴定含14-3-3蛋白抑制肽R18的重组腺病毒,为研究14-3-3蛋白的功能提供基础工具。方法:用同源重组方法构建含14-3-3蛋白抑制肽R18的复制缺陷型腺病毒载体(AdR18),并加以鉴定、扩增,以获得高滴度AdR18病毒液,体外感染乳大鼠心肌细胞,检测目的基因表达。结果:将构建的重组腺病毒载体AdR18感染乳大鼠心肌细胞并表达48h后,蛋白印迹结果显示AdR18感染组有明显R18的表达,对照组无表达。结论:腺病毒载体可高效率导入外源基因在心肌细胞中高表达。
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| 关 键 词： | 重组腺病毒 14-3-3蛋白 心肌细胞 |
Preparation of R 18, an Inhibitory Peptide of 14-3-3 Protein, Recombinant Adenovirus and Its Expression in Cardiac Myocyte |
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| LIAO Wen-qian,PANG Yan,YU Chang-an,LI Geng,WEN Jian-yan,ZHANG Yi-guan,LI Xiao-hui.Preparation of R 18, an Inhibitory Peptide of 14-3-3 Protein, Recombinant Adenovirus and Its Expression in Cardiac Myocyte[J].Progress in Modern Biomedicine,2008,8(11). |
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| Authors: | LIAO Wen-qian PANG Yan YU Chang-an LI Geng WEN Jian-yan ZHANG Yi-guan LI Xiao-hui |
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| Institution: | LIAO Wen-qiang~1 PANG Yan~2 YU Chang-an~1 LI Geng~1 WEN Jian-yan~1 ZHANG Yi-guan~2 LI Xiao-hui~2 |
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| Abstract: | Objective:To construct a recombinant adenovirus expressing R18,an inhibitory peptide of 14-3-3 protein.Methods: The adenovirus expressing R18 (AdR18) was prepared by the homorecomnination method,then identified and amplified.The high titer adnovirus product was used to infect neonatal cardiac myocyte,and the expression of R18 in cardiac myocyte was detected by PCR and Western blot.Results:48 hours after infected,the expression of R18 was detected in the AdR18 transfected group by western blot assay, but it was not found in the control Ad group.Conclusion:Adenovirus can high efficiently induce the expression of exogene in cardiac myocyte. |
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| Keywords: | Recombinant adenovirus 14-3-3 protein Cardiac myocyte |
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