Proteolytic enzymes in green wheat-leaves III. Inactivation of acid proteinase II by diazoacetyl-DL-norleucine methyl ester and 1,2-epoxy-3-(p-nitrophenoxy)-propane |
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| Authors: | Frith G J T; Peoples M B; Dalling M J |
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| Institution: | Plant Sciences Section, School of Agriculture and Forestry, University of Melbourne Parkville. Victoria. Australia. 3052 |
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| Abstract: | Acid proteinase II isolated from green wheat leaves in a purifiedform was rapidly inactivated at pH=5.5 to 6.0 by a 50-fold molarexcess of diazoacetyl-DL-norleucine methyl ester (DAN) in thepresence of cupric ions which were essential for inactivation.The acid proteinase was also inactivated by reaction with 1,2-epoxy-3-(p-nitrophenoxy)-propane(EPNP). The inactivation by EPNP was much slower than by DANand the half-life of the activity was 24 hr. (Received February 6, 1978; ) |
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