A general method for the transfer of plasmid-borne mutant alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda: transfer of pqq genes |
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| Authors: | J J M Meulenberg W A M Loenen E Sellink and P W Postma |
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| Institution: | (1) E.C. Slater Institute for Biochemical Research, and Biotechnology Center, University of Amsterdam, P.O. Box 20151, NL-1000 HD Amsterdam, The Netherlands;(2) Present address: The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands |
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| Abstract: | Summary A generally applicable method is described for reintroduction of mutant plasmid-borne alleles to the chromosome of Klebsiella pneumoniae using bacteriophage  . We, used this method to make stable chromosomal transposon insertions in genes for biosynthesis of pyrroloquinoline quinone in K. pneumoniae |
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| Keywords: | Transposon mutations Gene transfer Recombination pqq genes Klebsiella pneumoniae |
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