| Affiliation: | (1) Sezione di Genetica Medica, Dipartimento di Medicina Interna e del Lavoro, Policlinico, Piazza Giulio Cesare, I-70124 Bari, Italy e-mail: genmed@tin.it, Fax: +39-80-5478269, IT;(2) I.R.C.C.S. “De Bellis”, Castellana, Bari, Italy, IT;(3) Dipartimento di Medicina Interna,Università, Genoa, Italy, IT;(4) Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy, IT;(5) Istituto Nazionale Tumori, Milan, Italy, IT;(6) Ospedale Mauriziano, Turin, Italy, IT;(7) Cattedra di Genetica Medica, Dipartimento di Pediatria, Università di Padova, Padua, Italy, IT;(8) Dipartimento di Scienze Cliniche e Biologiche, Università di Pavia, Sede Varese, Italy, IT;(9) Dipartimento di Biochimica e Biotecnologie Mediche, Università Federico II, Naples, Italy, IT;(10) V Divisione e Cattedra di Chirurgia Generale, Policlinico, Bari, Italy, IT;(11) IV Divisione e Cattedra di Chirurgia Generale, Policlinico, Bari, Italy, IT |
| Abstract: | In the present study, we used five different polymorphic markers to construct the haplotype at the adenomatous polyposis coli (APC) locus in families with familial adenomatous polyposis (FAP) and in the normal Italian population. Non-ambiguous haplotypes were reconstructed from 246 normal chromosomes and 65 FAP chromosomes. In the control population, the four polymorphisms intragenic to APC gave rise to 16 haplotypes, the most common of which (II and XV) accounted for over 50% of all chromosomes. In FAP patients, 13 haplotypes were found but their distribution was not statistically different from normal subjects. Eighty complete chromosomal haplotypes (many fewer than the theoretical maximum of 208) for the five polymorphic sites assayed were observed in the control population, 35 being found in the FAP patients. We compared the distribution of these haplotypes within the two groups; no statistically significant differences between normal and FAP chromosomes were found. The elevated heterogeneity of FAP chromosomes was clearly confirmed by the observation that 19 patients who carried one or other of the two most common APC mutations (nt 3183 and nt 3927) showed 18 different haplotypes. On the basis of these results, we were not able to identify a founder FAP chromosome. Various mechanisms are presented to explain this observation. Received: 5 November 1997 / Accepted: 3 February 1998 |
