Identification of new low-molecular-weight glutenin subunit genes in wheat |
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Authors: | T M Ikeda T Nagamine H Fukuoka H Yano |
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Institution: | (1) National Agricultural Research Center for Western Region, 6-12-1 Nishifukatsu, Fukuyama, Hiroshima 721-8514, Japan e-mail: tmikeda@affrc.go.jp Tel.: +81-849-23-4100 ext.231, Fax: +81-849-24-7893, JP |
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Abstract: | To clarify the composition of low-molecular-weight glutenin subunits (LMW-GSs) in a soft wheat cultivar, we cloned and characterized
LMW-GS genes from a cDNA library and genomic DNA in Norin 61. Based on alignment of the conserved N- and C- terminal domains
of the deduced amino-acid sequences, these genes are classified into 12 groups. One of these groups (group 5), the corresponding
gene of which has not been reported previously, contains two additional hydrophobic amino-acid clusters interrupting the N-terminal
repetitive domain. Other groups (groups 11 and 12), which were not identified in other cultivars as a protein product, showed
all eight cysteines in the C-terminal conserved domain. With specific primer sets for these groups it was revealed that Glu-D3 and Glu-A3 encoded the former and the latter, respectively. Both groups of genes were expressed in immature seeds. The presence of these
groups of LMW-GSs may affect the dough strength of soft wheat.
Received: 26 March 2001 / Accepted: 16 July 2001 |
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Keywords: | Soft wheat Low-molecular-weight glutenin subunits Low-molecular-weight glutenin subunit genes Triticum aestivum |
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