| Abstract: | ![]()
Rodent ovariectomy is an experimental method to eliminate the main source of sexualsteroids. This work explored for the first time the ovariectomy temporal changes inducedin the hemostatic coagulation markers: prothrombin time (PT), activated partialthromboplastin time (aPTT), thrombin time (TT), and fibrinogen concentration (FIB) alongwith uterine weight on adult female CD1 mice and Wistar rats. Uterine weight (Uw) wasassessed before ovariectomy (control), and 1, 3, 5, 7, 9, 16, and 21 days after surgery.PT, aPTT, TT and FIB were estimated the same days, using reported standard techniques.Ovariectomy decreased Uw, since day 1; and from day 10 to 21 reached the lowest values forboth species. After day 1, mice hemostatic parameters changed (PT +10%,P<0.05; aPTT +53%, P<0.05; TT −24%,P<0.05; FIB +67%, P<0.05). Rats showedsignificant changes only in TT and FIB (TT −13%, P<0.001; FIB +65%,P<0.001). Neither mice PT, aPTT and TT, recovered control valuesafter 21 days. In the rats from day 5 to 16 aPTT diminished (18–23%,P<0.05) recovering to control values on day 21, TT after 9 days and PTon day 16. In both species, FIB returned to its control values after 9 days. Ovariectomydifferentially altered the PT hemostatic parameter of mice and rats indicating anon-equivalence among both species behaviour for experimental studies of bloodcoagulation. |
