The Escherichia coli dnaW mutation is an allele of the adk gene |
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| Authors: | Joan M Henson Aleksandra Blinkowa and James R Walker |
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| Institution: | (1) Department of Microbiology, University of Texas, 78712-1095 Austin, Texas, USA;(2) Present address: Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Cotorado, USA;(3) Present address: Institute of Microbiology, University of Warsaw, Warsax, Poland |
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| Abstract: | Summary A dnaW mutant, isolated on the basis of inability to effect conjugal DNA transfer at high temperature, has been shown by complementation and enzyme assay to be defective in the adk (adenylate kinase; EC 2.7.4.3) locus. The adk mutant, known to have reduced ATP concentration at the nonpermissive temperature (Cousin and Belaich 1966), was used to demonstrate a donor energy requirement for stable aggregate formation and for chromosome transfer in conjugation. |
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