d-Hydantoinase from anaerobic microorganisms |
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| Authors: | André Morin Jean-Pierre Touzel Alain Lafond Danielle Leblanc |
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| Affiliation: | (1) Food Research and Development Centre, 3600, Casavant Boulevard West, J2S 8E3 St. Hyacinthe, Québec, Canada;(2) Campus Haute-technologie Agro-alimentaire de Saint-Hyacinthe Inc., 780, avenue de l'Hôtel de Ville, J2S 5B2 Saint Hyacinthe, Québec, Canada;(3) Present address: INRA, 369 rue Jules Guesde, B. P. 39, F-59651 Villeneuve d'Ascq Cedex, France |
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| Abstract: | Summary Of 373 anaerobic microbial isolates screened for the enzymatic conversion of dihydrouracil to N-carbamyl- -alanine, several strains of Clostridium spp., C. glycolicum, C. subterminale and Peptococcus anaerobius were positive. These Clostridium and Peptococcus strains produced also N-carbamyl-d-amino acids from the respective 5 -monosubstituted hydantoins. The d-hydantoinase activity from whole cell suspensions of P. anaerobius strain CRDA 303 was characterized with regard to pH and temperature stability and activity by using dihydrouracil (DHU) and isopropylhydantoin (IPH) as substrates. The d-hydantoinase from P. anaerobius was optimal at 60°C and at pH 6.5–9.5 for the substrate DHU. It was stable up to 55°C and at pH 5.0–9.5 and could be stored at 4°C under an aerobic atmosphere for at least 14 days.Offprint requests to: A. Morin |
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