灌浆期水稻受褐飞虱侵害后赤霉素含量的变化

应用酶联免疫吸附法(ELISA)研究灌浆期水稻协优963、TN1受褐飞虱Nilaparvata lugens(Stl)若虫侵害后根、叶片赤霉素(gibberellin,GA)含量的变化,以探讨褐飞虱若虫侵害后灌浆期水稻耐虫性与植物体内源激素的关系。结果表明:虫口密度40头/株和80头/株120头/株侵害灌浆期水稻协优963后3d根GA含量显著下降,40头/株和80头/株侵害后协优963后3d根冠(叶片)比显著下降;40头/株侵害协优963后6d根冠比显著下降。40头/株、80头/株和120头/株侵害灌浆期水稻TN1后3d根GA含量显著下降;80头/株和120头/株侵害TN1后6d根GA含量显著下降,120头/株侵害TN1后6d根冠比显著下降。表明灌浆期水稻协优963、TN1受褐飞虱若虫侵害后基本变化趋势为根GA含量、GA含量根冠比下降(3d、6d),叶片GA含量变化规律不明显;耐、感虫水稻品种变化一致,变化幅度无明显差异。

Effects of Nilaparvata lugens infestation on gibberellin levels of rice at the grain-filling stage

To understand the relationship between tolerance and endogenous plant hormones in rice plants infested with Nilaparvata lugens （Still） at the grain-filling stage, change in gibberellin （GA） in the roots and leaves of two representative rice varieties in response to N. lugens nymph infestation was studied using enzyme-linked immunosorbent assay （ELISA）. The results indicate that GA content in the roots of the Xieyou 963 variety, and the ratio of GA contents in roots to that in leaves （R/L ratio） , decreased significantly after 3 d, and the R/L ratio also decreased significantly after 6 d. The GA content of the roots of the TNlvariety also decreased significantly after 3 d and 6 d and the R/L ratio decreased after 6 d. These results show that the GA content of roots and the R/L ratio of both tolerant and susceptible rice varieties decreased at 3 d and 6 d after N. lugens infestation during the grain-filling stage. The GA content of leaves did not, however, change significantly. So the two rice varieties had the same change law, no significant difference.