Evaluation of simple enzyme kinetics by response surface modelling |
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| Authors: | Mats Andersson Patrick Adlercreutz |
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| Affiliation: | (1) Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. 124, S-22100 Lund, Sweden |
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| Abstract: | The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined. |
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| Keywords: | alcohol dehydrogenase cyclohexanone enzyme kinetics response surface modelling |
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