Enzymatic evidence for involvement of the methylmalonyl-CoA pathway in propionate oxidation by Syntrophobacter wolinii |
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| Authors: | Frans P Houwen Jeannette Plokker Alfons J M Stams Alexander J B Zehnder |
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| Institution: | (1) Department of Microbiology, Agricultural University of Wageningen, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands;(2) Present address: Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, S-750 07 Uppsala, Sweden |
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| Abstract: | Enzyme measurements were carried out with crude cell-free extracts of the propionate oxidizing coculture of Syntrophobacter wolinii and Desulfovibrio G11. Using cell-free extracts of a pure culture of Desulfovibrio G11 as a blank, most of the enzymes involved in the methylmalonyl-CoA pathway for propionate oxidation, including a propionyl-CoA: oxaloacetate transcarboxylase, were demonstrated in S. wolinii. |
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| Keywords: | Syntrophobacter wolinii Propionate oxidation Syntrophic coculture Propionyl-CoA: oxaloacetate transcarboxylase Methylmalonyl-CoA pathway Desulfovibrio |
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