Human dermal fibroblasts express multiple bFGF and aFGF proteins |
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Authors: | Leslie L Root Gary D Shipley |
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Institution: | (1) Department of Cell Biology and Anatomy, the Oregon Health Sciences University, 97201 Portland, Oregon |
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Abstract: | Summary We investigated the regulation of expression of bFGF and aFGF in cultures of normal human dermal fibroblasts grown in a defined,
serum-free medium which did not contain FGF. Under these conditions we detected three molecular weight forms of bFGF protein
18.0, 23.0, and 26.6 kiloDaltons (kD)] and three molecular weight forms of aFGF protein (18.4, 19.2, and 28.6 kD) in these
cells using western blot analysis. The addition of fetal bovine serum (FBS) to these cultures caused an accumulation of all
three molecular weight forms of bFGF protein with a more dramatic accumulation of the 23.0 and 26.6 kD forms. In contrast,
the addition of FBS to the cultures had no effect on the level of aFGF proteins. Analysis of mRNA isolated from cells grown
in serum-free medium revealed multiple species of both bFGF and aFGF RNA with molecular weights that correlated with our previous
observations. The abundance of all bFGF mRNA species increased dramatically after serum treatment while the abundance of aFGF
mRNA species increased only slightly. Our observations demonstrate that factor(s) present in FBS elevate the levels of bFGF
mRNA and protein beyond the levels already present in the cultures growing in serum-free medium. Moreover, both bFGF and aFGF
protein are present in these cells as multiple molecular weight species. Some of these forms are higher in apparent molecular
weight than would be predicted from ATG-initiated primary translation products of these genes. We also show that the cells
used for this study proliferate in response to bFGF and aFGF, thus, it is possible that the growth of these cells could be
subject to autocrine/paracrine control in certain conditions. |
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Keywords: | bFGF aFGF human dermal fibroblasts serum-free medium |
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