Thyroid hormone stimulates expression of 6-phosphofructo-2-kinase in rat liver

Gel-filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin-induced increase in cytoplasmic pH (pH_i) in BCECF-loaded platelets. This rise in pH_i was strongly inhibited by the Na⁺/H⁺ exchange blocker EIPA. The potentiation by adrenaline of thrombin-induced PLC activation measured as ³²P]PA formation and final platelet responses was, however, not blocked by EIPA, even at low concentrations of thrombin. These results indicate that the enhancement by adrenaline of thrombin-induced cytoplasmic alkalinization may be a secondary effect which is not essential for the potentiation by adrenaline of platelet activation by thrombin.