Mycoplasma provides for the spreading of opossum kidney cells in a serum-free,defined medium |
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Authors: | Lisa J Leiderman Vincent W Dennis |
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Institution: | (1) Department of Medicine, Division of Nephrology, Duke University Medical Center, P.O. Box 3014, 27710 Durham, North Carolina;(2) Amgen, 1900 Oak Terrace Lane, 91320 Thousand Oaks, California |
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Abstract: | Summary During the course of investigating the growth and differentiation of opossum kidney cells in serum-free medium, it was observed
that a mycoplasma contamination (M. hyorhinis) contributed to the spreading of cells. Contaminated cells, seeded on collagen-coated plates, spread out and grew to confluency
in Dulbecco’s modified eagle’s medium/Ham’s F12 nutrient mixture containing insulin, transferrin, sodium selenite, and bovine
serum albumin fraction V. In addition, differentiated characteristics, including parathyroid hormone-inhibitable, sodium-dependent
phosphate transport, were expressed by these cells grown in this medium. After the infection was eradicated, the contamination-free
cells would not spread out and proliferate in the same serum-free medium as they had done in the presence of mycoplasma. Normal
cellular development, however, was attained after cells were plated in serum-free medium that included fetuin. Cells once
again spread out, grew to confluency, and were able to express their differentiated characteristics. |
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Keywords: | opossum kidney mycoplasma spreading factor serum-free medium fetuin |
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