Purification and characterisation of periplasmic C-type cytochromes from Desulfovibrio desulfuricans (NCIMB 8372) |
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Authors: | Lars H Eng Halina Y Neujahr |
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Institution: | (1) Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-100 44 Stockholm, Sweden |
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Abstract: | Periplasmic extract from Desulfovibrio desulfuricans (NCIMB 8372) was found to contain two different c-type cytochromes. One is tetraheme cytochrome c3 and the other is monoheme cytochrome c553. Cytochrome c3 could be purified by a procedure involving only one chromatographic step, whereas cytochrome c553 required several such steps. Cytochrome c3 was found to have a relative molecular mass of 14300 and an isoionic point higher than 9. Analysis of the redox potentials indicated one heme at -260 mV and three hemes around -330 mV. Cytochrome c553 had a relative molecular mass of 7200, an isoionic point higher than 9 and a redox potential of 0 mV. |
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Keywords: | Desulfovibrio Cytochrome c3 Cytochrome c553 Periplasm Redox potentials |
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