Chromosomal location of rDNA in Allium: in situ hybridization using biotin- and fluorescein-labelled probe |
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| Authors: | A Ricroch E B Peffley R J Baker |
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| Institution: | (1) Department of Agronomy, Horticulture, and Entomology and the Institute of Biotechnology, Texas Tech University, 79409-2134 Lubbock, TX, USA;(2) Department of Biological Sciences and the Institute of Biotechnology, Texas Tech University, 79409-3131 Lubbock, TX, USA;(3) Present address: Laboratory of Plant Evolution and Systematics Bldg, University of Paris XI Orsay, 362, F-91405 Orsay Cedex, France |
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| Abstract: | Summary A biotin- and fluorescein-labelled probe of Helianthus argophyllus has been used to map specific repeated rDNA sequences by in situ hybridization on mitotic chromosomes of Alliwn cepa, Allium fistulosum, a diploid interspecific (Allium fistulosum x Allium cepa) F1 hybrid, and a triploid interspecific (2 x = A. cepa, 1 x = A. fistulosum) shallot. Hybridization sites were restricted to satellited and smallest pairs of chromosomes in both A. cepa and A. fistulosum. The number, size, and position of the hybridization sites distinguish homologous chromosomes and identify the individual chromosomes carrying the nucleolus organizing region (NOR) at the secondary constriction, as well as the individual chromosomes carrying an additional NOR. This in situ hybridization technique is the first reported in a plant species and offers new cytogenetic markers in Allium. |
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| Keywords: | Chromosomes Introgression Karyotypic analysis Non radioactive labelling NOR regions |
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