Morphologic evaluation and expression of matrix metalloproteinases-2 and 9 and nitric oxide during experimental periodontal disease in rat |
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Authors: | Camila Oliveira Rodini Aline Carvalho Batista Thiago José Dionísio Carlos Ferreira Santos Fernando Queiroz Cunha Vanessa Soares Lara |
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Institution: | 1.Department of Stomatology (Oral Pathology), Bauru School of Dentistry,University of S?o Paulo,Sao Paulo,Brazil;2.Bauru,Brazil;3.Department of Stomatology (Oral Pathology), School of Dentistry,Federal University of Goiás,Goiania,Brazil;4.Department of Biological Sciences (Pharmacology), Bauru School of Dentistry,University of S?o Paulo,Sao Paulo,Brazil;5.Department of Pharmacology, Faculty of Medicine of Ribeir?o Preto,University of S?o Paulo,Ribeirao Preto,Brazil |
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Abstract: | The immunopathologic and inflammatory mechanisms involved in periodontal disease (PD) include the participation of host resident,
inflammatory cells and chemical mediators. Metalloproteinases (MMPs) and nitric oxide (NO) play essential role in extracellular
matrix turnover of periodontal tissue destruction. In this study, by means of RT-PCR through semi-quantitative densitometric
scanning methods, the expression of MMPs -2 and -9 and inducible NO synthase (iNOS) was temporally and spatially investigated
during the destructive mechanisms of experimentally induced PD in rats. Samples from different periods were microscopically
analyzed and compared with the contralateral side (control). Our results showed significant expression of MMP-9 and iNOS in
tissues affected by PD, as compared with controls, three days after PD induction, simultaneously with the beginning of alveolar
bone loss. At 7 days post induction, only the MMP-9 mRNA presented a significantly higher expression, as compared with the
respective controls. Thus, in the rat ligature-induced PD, MMP-9 and iNOS might importantly participate in the early stages
of the disease, including inflammatory cell migration, tissue destruction and alveolar bone resorption. Also, we may suggest
that the exuberant presence of PMNs may be related to the important expression of iNOS and MMP-9 found at 3 days post induction. |
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Keywords: | Periodontal disease MMP-2 MMP-9 iNOS |
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