Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples |
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| Authors: | Karanis Panagiotis Thekisoe Oriel Kiouptsi Klytaimnistra Ongerth Jerry Igarashi Ikuo Inoue Noborou |
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| Affiliation: | National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan. Panagiotis.Karanis@uk-koeln.de |
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| Abstract: | A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence. |
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