| 链霉菌的rep-PCR基因指纹分析 |
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| 引用本文： | 张建丽,刘志恒.链霉菌的rep-PCR基因指纹分析[J].微生物学报,2004,44(3):281-285. |
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| 作者姓名： | 张建丽 刘志恒 |
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| 作者单位： | 1. 北京理工大学,生命科学与技术学院,北京,100081
2. 中国科学院微生物研究所,微生物资源国家重点实验室,北京,100080 |
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| 基金项目： | 北京理工大学基础研究基金资助项目 (BIT_UBF_2 0 0 3 0 6C0 8)~~ |
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| 摘 要： | 对重复片段PCR(repPCR)基因指纹分析应用于链霉菌分子分型进行研究，结果表明repPCR基因指纹分析具有分辨率高、稳定、重现性好、简便易行等特点，在一定程度上与16S rDNA 序列比较结果相一致，是一种快速而有效的DNA指纹技术，能反映出链霉菌种和菌株水平的基因型、系统发育和分类学关系，可应用于种及以下水平的分类和快速鉴定，尤其适用于分析大量的菌株或分离株。
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| 关 键 词： | 链霉菌，repPCR基因指纹分析，快速鉴定 |
| 文章编号： | 0001-6209(2004)03-0281-05 |
| 修稿时间： | 2003年7月18日 |
Repetitive-element PCR Genomic Fingerprinting of The Genus Streptomyces |
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| ZHANG Jian,Li LIU Zhi-Heng.Repetitive-element PCR Genomic Fingerprinting of The Genus Streptomyces[J].Acta Microbiologica Sinica,2004,44(3):281-285. |
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| Authors: | ZHANG Jian Li LIU Zhi-Heng |
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| Institution: | ZHANG Jian Li1 LIU Zhi-Heng2 |
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| Abstract: | Repetitive element PCR (rep PCR) genomic fingerprinting with the BOXA1R primer was investigated as a molecular typing tool for strains of Streptomyces. The results indicated that rep PCR DNA fingerprints of Streptomyces strains were relatively unique, simple, stable, and reproducible. To some extent, rep PCR generated genomic fingerprint analyses yield results that are in agreement with 16S rDNA sequences study. This method appears to reflect the genotypic, phylogenetic and taxonomic relationships of the genus Streptomyces in the species strain level. Therefore, it can be used as a rapid and efficient means of determining taxonomic diversity and phylogenetic structure, especially of large collections of strains. |
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| Keywords: | Streptomyces Repetitive-element PCR genomic fingerprinting Rapid identification |
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