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Autofluorescence and Ultrastructure in the Myxomycete Diachea leucopodia (Physarales)
Authors:Cecilia C Carmarán  Sonia Rosenfeldt  Diana Skigin  Marina Inchaussandague  Harold W Keller
Institution:1. Departamento de Biodiversidad y Biología Experimental, FCEN, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, C1428EHA, Buenos Aires, Argentina
2. Grupo de Electromagnetismo Aplicado, Departamento de Física, FCEN, Ciudad Universitaria, Universidad de Buenos Aires, and IFIBA, CONICET, Pabellón I, C1428EHA, Buenos Aires, Argentina
3. Department of Biology and Earth Science, University of Central Missouri, Warrensburg, MO, 64093, USA
4. Botanical Research Institute of Texas, Fort Worth, TX, 76107, USA
Abstract:Autofluorescence is reported for the first time in Myxomycete fruiting bodies. Ultrastructure of stalked sporangia of Diachea leucopodia (Didymiaceae, Physarales) was studied using scanning and transmission electron microscopy, energy-dispersive X-ray microanalysis, and fluorescence microscopy. External and internal properties of the peridium that surround the spores and capillitium exhibit autofluorescence. The stalk is composed of calcareous granules and energy-dispersive X-ray microanalysis demonstrates that the elemental composition of the peridium, capillitium, and stalk has varying concentrations of calcium.
Keywords:
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