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Improvement of Manganese Peroxidase Production by the Hyper Lignin-Degrading Fungus Phanerochaete sordida YK-624 by Recombinant Expression of the 5-Aminolevulinic Acid Synthase Gene |
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| Authors: | Hirofumi Hirai Kenta Misumi Tomohiro Suzuki Hirokazu Kawagishi |
| Institution: | 1. Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan 2. Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan 3. Graduate School of Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan |
| Abstract: | The manganese peroxidase (MnP) gene (mnp4) promoter of Phanerochaete sordida YK-624 was used to drive expression of 5-aminolevulinic acid synthase (als), which is a key heme biosynthesis enzyme. The expression plasmid pMnP4pro-als was transformed into P. sordida YK-624 uracil auxotrophic mutant UV-64, and 14 recombinant als expressing-transformants were generated. Average cumulative MnP activities in the transformants were 1.18-fold higher than that of control transformants. In particular, transformants A-14 and A-61 showed significantly higher MnP activity (approximately 2.8-fold) than wild type. RT-PCR analysis indicated that the increased MnP activity was caused by elevated recombinant als expression. These results suggest that the production of MnP is improved by high expression of als. |
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