Inoculation and nitrate alter phytohormone levels in soybean roots: differences between a supernodulating mutant and the wild type |
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Authors: | Juan M Caba M Luz Centeno Belén Fernández Peter M Gresshoff Francisco Ligero |
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Institution: | (1) Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain, ES;(2) Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, 33071 Oviedo, Spain, ES;(3) Department of Botany, The University of Queensland, Brisbane, St. Lucia Qld 4072, Australia, AU |
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Abstract: | The levels of different cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) in roots of Glycine max L.] Merr. cv. Bragg and its supernodulating mutant nts382 were compared for the first time. Forty-eight hours after inoculation with Bradyrhizobium, quantitative and qualitative differences were found in the root's endogenous hormone status between cultivar Bragg and the
mutant nts382. The six quantified cytokinins, ranking similarly in each genotype, were present at higher concentrations (30–196% on average
for isopentenyl adenosine and dihydrozeatin riboside, respectively) in mutant roots. By contrast, the ABA content was 2-fold
higher in Bragg, while the basal levels of IAA 0.53 μmol (g DW)−1, on average] were similar in both genotypes. In 1 mM NO3
−-fed Bragg roots 48 h post-inoculation, IAA, ABA and the cytokinins isopentenyl adenine, and isopentenyl adenosine quantitatively
increased with respect to uninoculated controls. However, only the two cytokinins increased in the mutant. High NO3
− (8 mM) markedly reduced root auxin concentration, and neither genotypic differences nor the inoculation-induced increase
in auxin concentration in Bragg was observed under these conditions. Cytokinins and ABA, on the other hand, were little affected
by 8 mM NO3
−. Root IAA/cytokinin and ABA/cytokinin ratios were always higher in Bragg relative to the mutant, and responded to inoculation
(mainly in Bragg) and nitrate (both genotypes). The overall results are consistent with the auxin-burst-control hypothesis
for the explanation of autoregulation and supernodulation in soybean. However, they are still inconclusive with respect to
the inhibitory effect of NO3
−.
Received: 16 April 1999 / Accepted: 13 December 1999 |
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Keywords: | : Abscisic acid – Auxin – Cytokinin – Glycine (soybean) – Modulation – Nodulation mutant |
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