黄毛纺蚋线粒体基因组全序列测定与分析

利用PCR步移法对黄毛纺蚋的线粒体基因组全序列进行了测定和分析。黄毛纺蚋线粒体基因组全长15904 bp(Gen Bank序列号KP793690),包括13个蛋白编码基因、22个tRNA基因、2个rRNA基因以及长度为939 bp的非编码区。A、T、C、G碱基含量分别为39.1%、35.8%、10.4%、14.7%。9个蛋白编码基因和14个tRNA基因在J链编码,其余4个蛋白编码基因和8个tRNA基因在N链编码,基因排列顺序与其它已知双翅目昆虫相同。13个蛋白编码基因中除COI以TTG作为起始密码外,其余蛋白质基因均以ATN作为起始密码子,终止密码子多数为典型的TAA、TAG,只有COI和ND4L以单独的T作为终止密码子。在所测得的22个tRNA基因中,除tRNASer(AGN)缺少DHU臂外,其余tRNA均能形成典型的三叶草结构。

Sequence and analysis of complete mitochondrial genome of Simuiium aureohirtum

The complete mitochondrial genome of Simulium aureohirtum was sequenced and analyzed by using PCR amplification and prime walking. The complete mitochondrial genome of S. Saureohirtum is a circular molecule of 15904 nucleotides (GenBank Accession No. KP793690), including 22 tRNA genes, 2 rRNA genes, 13 protein-coding genes and 939 bp non-coding region. The nucleotide composition of A,T, C, and G were 39. 1%, 35.8%, 10.4%, and 14.7%, respectivelyNine protein-coding genes and 14 tRNA genes were encoded on the J strand, and the other 4 protein-coding genes and 8 tRNA genes were encoded on the N strand. The arrangement of genes in S. Aureohirtum is consistent with that of other Diptera speicesExcept the initiation codons of COI gene is a TTG codon, the other protein coding genes start with a typical ATN codonMost of the 13 PCGs in S.aureohirtum have complete termination codons (TAA, TAG), except the termination codons in COI and ND4 that have incomplete TTwenty one tRNAs have typical cloverleaf structure, except for tRNA Ser (AGN) without the dihydrouridine (DHU).