Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings |
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| Authors: | Christine M Roche Clare J Dibble and Jonathan J Stickel |
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| Institution: | (1) National Renewable Energy Laboratory, National Bioenergy Center, 1617 Cole Boulevard, Golden, CO 80401-3393, USA;(2) Department of Chemical Engineering, University of California-Berkeley, 201 Gilman Hall, Berkeley, CA 94720-1462, USA |
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| Abstract: | Background Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor
in the development of economically viable lignocellulosic ethanol. Shake flasks, the reaction vessel commonly used for screening
enzymatic saccharifications of cellulosic biomass, do not provide adequate mixing at high-solids concentrations when shaking
is not supplemented with hand mixing. |
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