Protein Bodies in the Pine Megagametophyte in vitro Culture: Ultrastructural, Histochemical and Electrophoretic Observations期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Protein Bodies in the Pine Megagametophyte in vitro Culture: Ultrastructural, Histochemical and Electrophoretic Observations

Authors:	J. Hřib R. Janisch B. Vooková J. Nahálková P. Gemeiner M. Hajduch

Affiliation:	(1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra, Slovakia;(2) Department of Biology, Faculty of Medicine, Masaryk University, Jotova 10, 662 43 Brno, Czech Republic e-mail;(3) Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovakia

Abstract:	The megagametophytes of the European black pine (Pinus nigra Arn.) were cultured on modified MS medium. After 10 d, protein bodies showed well-marked degradation on freeze-etched replicas and in preparations observed by scanning electron microscopy. After 20 d of cultivation, the megagametophyte cells were completely empty. Proteins secreted into the agar medium were determined by electrophoresis and 15 different proteins, in the range of 6.5 to 71 kDa, were identified.

Keywords:	Pinus nigra protein bodies degradation proteins SDS-PAGE
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