Protein Bodies in the Pine Megagametophyte in vitro Culture: Ultrastructural, Histochemical and Electrophoretic Observations |
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| Authors: | J H?ib R Janisch B Vooková J Nahálková P Gemeiner M Hajduch |
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| Institution: | (1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra, Slovakia;(2) Department of Biology, Faculty of Medicine, Masaryk University, Jo tova 10, 662 43 Brno, Czech Republic e-mail;(3) Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovakia |
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| Abstract: | The megagametophytes of the European black pine (Pinus nigra Arn.) were cultured on modified MS medium. After 10 d, protein bodies showed well-marked degradation on freeze-etched replicas and in preparations observed by scanning electron microscopy. After 20 d of cultivation, the megagametophyte cells were completely empty. Proteins secreted into the agar medium were determined by electrophoresis and 15 different proteins, in the range of 6.5 to 71 kDa, were identified. |
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| Keywords: | Pinus nigra protein bodies degradation proteins SDS-PAGE |
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