Enigmatic double-stranded RNA in Japonica rice |
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| Authors: | Toshiyuki Fukuhara Hiromitu Moriyama JunYong Pak Hiroshi Hyakutake Takeshi Nitta |
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| Institution: | (1) Laboratory of Biology, Faculty of General Education, Tokyo University of Agriculture & Technology, 183 Fuchu, Tokyo, Japan;(2) Laboratory of Biology, Korea University, 187 Kodaira, Tokyo, Japan;(3) Department of Chemical Regulation of Biomechanisms, The Institute of Physical & Chemical Research, 2-1, Hirosawa, 351-01 Wako, Saitama, Japan |
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| Abstract: | We have found a linear, 16 kb, double-stranded RNA (dsRNA) in symptomless Japonica rice (Oryza sativa L.) that is not found in Indica rice (Oryza sativa L.). The dsRNA was detected in every tissue and at every developmental stage, and its copy number was approximately constant (about 20 copies/cell). Double-stranded RNA was also detected in two strains of Oryza rufipogon (an ancestor of O. sativa). Hybridization experiments indicated that the dsRNA of O. rufipogon was homologous but not identical to that of O. sativa. The sequence of about 13.2 kb of the dsRNA was determined and two open reading frames (ORFs) were found. The larger ORF (ORF B) was more than 12 351 nucleotides long and encoded more than 4 117 amino acid residues. |
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| Keywords: | double-stranded RNA Japonica rice Oryza rufipogon Oryza sativa L RNA plasmid |
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