Subdoligranulum variabile gen. nov., sp. nov. from human feces |
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| Authors: | Holmstrøm Kim Collins Matthew D Møller Trine Falsen Enevold Lawson Paul A |
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| Institution: | 1. Bioneer A/S, Department of Molecular Characterization, Kogle Alle 2, DK-2970 Hørsholm, Denmark;2. School of Food Biosciences, University of Reading, Reading RG6 6AP, UK;3. CCUG Collection, Department of Clinical Bacteriology, S-413 46 Göteborg, Sweden;1. BK21PLUS Program in Embodiment: Health-Society Interaction, Department of Public Health Sciences, Graduate School, Korea University, Seoul, Republic of Korea;2. Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Republic of Korea;3. ChunLab, Inc., Seoul, Republic of Korea;4. School of Biosystem and Biomedical Science, Korea University, Seoul, Republic of Korea;1. TI Food and Nutrition, Wageningen, The Netherlands;2. Laboratory of Neurogastroenterology, Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland;3. Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Sadat City, Egypt;4. Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands;5. Host-Microbe Interactomics Group, Wageningen University, Wageningen, The Netherlands;6. NIZO Food Research, Ede, The Netherlands;1. Jill Roberts Institute for Research in IBD, Weill Cornell Medicine, New York, New York;2. Division of Gastroenterology and Hepatology, New York Presbyterian Hospital–Weill Cornell Medical Center, New York, New York;3. St. Mary’s Hospital, Department of Medicine, Waterbury, Connecticut;4. Boehringer Ingelheim SHINE Program, Ridgefield, Connecticut;5. Finch Therapeutics, Somerville, Massachusetts;6. Jill Roberts Center for IBD, New York Presbyterian Hospital–Weill Cornell Medical Center, New York, New York;1. BGI-Shenzhen, Shenzhen, China;2. China National Genebank, Shenzhen, China;3. Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research, Shenzhen, China;4. Chinese PLA General Hospital, Beijing, China;5. Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, Shenzhen, China;6. Midea Group, Foshan, China;7. Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, Denmark;8. Qingdao-Europe Advanced Institute for Life Sciences, Qingdao, Shandong 266555, China;9. Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark;1. Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands;2. Caelus Pharmaceuticals, 3474 KG Zegveld, the Netherlands;3. Department of Agricultural Sciences, University of Naples “Federico II”, 80055 Portici, Italy;4. Proteomics & Mass Spectrometry Laboratory, ISPAAM - CNR, 80147 Naples, Italy;5. Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, the Netherlands;6. Istituto di Biostrutture e Bioimmagini IBB – CNR, I-80134 Naples, Italy;7. Food Quality & Design Group Wageningen University, Wageningen, the Netherlands;8. Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland;1. Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia;2. University of Bordeaux IBGC, UMR 509, France;3. Friedrich-Alexander-University of Erlangen-Nürnberg, Department of Chemistry and Pharmacy, Erlangen, Germany;4. University of Belgrade, Faculty of Biology, Centre for Electron Microscopy, Belgrade, Serbia |
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| Abstract: | During studies on the microflora of human feces we have isolated a strictly anaerobic, non-spore-forming, Gram-negative staining organism which exhibits a somewhat variable coccus-shaped morphology. Comparative 16S ribosomal RNA gene sequencing studies show the unidentified organism is phylogenetically a member of the Clostridium leptum supra-generic rRNA cluster and displays a close affinity to some rDNA clones derived from human and pig feces. The nearest named relatives of the unidentified isolate corresponded to Faecalibacterium prausnitzii (formerly Fusobacterium prausnitzii) displaying a 16S rRNA sequence divergence of approximately 9%, with Anaerofilum agile and A. pentosovorans the next closest relatives of the unidentified bacterium (sequence divergence approximately 10%). Based on phenotypic and phylogenetic considerations, it is proposed that the unusual coccoid-shaped organism be classified as a new genus and species, Subdoligranulum variabile. The type strain of S. variabile is BI 114(T) (=CCUG 47106(T)=DSM 15176(T)). |
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