Multiple shoot-bud formation and plantlet regeneration on Castanea sativa Mill. seeds in culture |
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Authors: | Roberto Rodríguez |
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Institution: | (1) Department of Chemistry and Biochemical Sciences, Oregon Graduate Center, 97006 Beaverton, OR;(2) Present address: Dpto. Fisiología Vegetal — Facultad de Ciencias Biológicas, Universidad de Oviedo, Oviedo, Spain |
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Abstract: | Primordial initiation and development of shoot-buds has been accomplished by using shoots derived from chestnut (Castanea sativa Mill) seedlings cultured with added 6-benzylaminopurine (BAP). Germination of chestnut seeds in the presence of BAP (4 – 40 M) stimulated varying numbers of shoot-buds in those areas of the main axis that were favorably altered. When excised single shoots from these treated seeds were subcultured on a fresh medium containing BAP (4 – 40 M) continual shoot production was observed. Bud growth and shoot elongation were stimulated by transferring cultures to a reduced concentration of BAP (2 M) plus indole-3-butyric acid (IBA 0.4 M). Plant regeneration occurred in the presence of IBA (0.8 M) after a preconditioning treatment in which naphthaleneacetic acid (NAA 50 M) and kinetin (k 2 M) were applied to the tissue culture shoots for 7 days in light. |
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