Capillaria hepatica: Granuloma formation to eggs: III. Anti-immunoglobulin augmentation and reagin activity in mice |
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Authors: | Richard Raybourne Gene B Solomon |
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Institution: | Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19174 USA |
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Abstract: | Sera from mice with primary and secondary Capillaria hepatica egg granulomas were examined for 48-hr homologous PCA activity and for the presence of IgM, IgG1, and IgG2 using a microtiter anti-globulin augmentation assay following indirect hemagglutination testing. All three antibody types assayed were present. Secondary granuloma formation produced an antibody response characterized by the initial production of IgM followed by IgG1 and IgG2 during the latter phase of the test period, however primary granulomatous sera demonstrated a more varied antibody response with the presence of all three during the entire test period. Forty-eight-hour PCA tests demonstrated the presence of reagin activity in sera from granulomatous and infected mice. Reagin activity occurred more frequently in primary than secondary granulomatous mice. These studies thus confirm the presence of antibody during granuloma formation to C. hepatica eggs and conclusively demonstrate the presence of at least two classes of antibody. |
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Keywords: | Granuloma formation Helminth egg Immunity cell-mediated humoral Indirect hemagglutination anti-globulin augmentation of Passive cutaneous anaphylaxis Mice |
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