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Two genes for the high mobility group protein HMG-Y are present in the genome of Canavalia gladiata D.C.
Authors:Yamamoto  Sumiko  Minamikawa  Takao
Affiliation:(1) Present address: Bionic Design Group, National Institute for Advanced Interdisciplinary Research, Tsukuba, Ibaraki, 305, Japan;(2) Department of Biology, Tokyo Metropolitan University, Minami-ohsawa, Hachioji, Tokyo, 192-03, Japan
Abstract:Two cDNAs encoding high-mobility-group (HMG) proteins that correspond to animal HMG-Y proteins were isolated from maturing seeds of Canavalia gladiata D.C. The deduced amino acid sequences of these cDNAs showed similarity to other plant HMG-I/Y proteins reported to date. The mRNAs for the HMG-Y proteins were detected in leaves, stems, roots, pods and seeds of C. gladiata. The level of the mRNA was high in the maturing seeds of 30 days after flowering and 2-day germinated seeds. Two genomic clones were isolated from DNA of C. gladiata and both were shown to represent single-copy genes consisting of two exons and one intron. This is the first report of the genomic sequences for HMG-I/Y protein in plants.
Keywords:A/T hook  A/T-rich sequence  Canavalia gladiata  GRP motif  high-mobility-group (HMG) protein  seed storage protein
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