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Antibiotics-free stable polyhydroxyalkanoate (PHA) production from carbon dioxide by recombinant cyanobacteria |
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| Authors: | Akiyama Hideo Okuhata Hiroshi Onizuka Takuo Kanai Shozo Hirano Masahiko Tanaka Satoshi Sasaki Ken Miyasaka Hitoshi |
| Institution: | aToray Research Center, Inc., 6-10-1 Tebiro, Kamakura, Kanagawa 248-8555, Japan;bThe Kansai Electric Power Co., Environmental Research Center, Keihanna-Plaza 12F, 1-7 Seikacho, Sourakugun, Kyoto 619-0237, Japan;cGraduate School of Engineering, Hiroshima Kokusai Gakuin University, 6-20-1 Nakano Aki-ku, Hiroshima 739-0321, Japan |
| Abstract: | A practical antibiotics-free plasmid expression system in cyanobacteria was developed by using the complementation of cyanobacterial recA null mutation with the EscherichiacolirecA gene on the plasmid. This system was applied to the production of polyhydroxyalkanoate (PHA), a biodegradable plastic, and the transgenic cyanobacteria stably maintained the pha genes for PHA production in the antibiotics-free medium, and accumulated up to 52% cell dry weight of PHA. |
| Keywords: | Cyanobacteria PHA recA Carbon dioxide Antibiotics-free |
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