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Direct and Continuous Electrochemical Measurement of Noradrenaline-Induced Nitric Oxide Production in C6 Glioma Cells |
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| Authors: | Trevin Stéphane Kataoka Yasufumi Kawachi Rikako Shuto Hideki Kumakura Konosuke Oishi Ryozo |
| Affiliation: | (1) ASTEC Head Office, 136-2 Minamizato, Shime-machi, Kasuya-gun, Fukuoka, 811-22, Japan;(2) Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-82, Japan;(3) Life Science Institute, Sophia University, 7-1 Kiyoi-cho, Chiyoda-ku, Tokyo, 102, Japan |
| Abstract: | 1. Nitric oxide (NO) production in C6 glioma cells was directly monitored in real time by electrochemical detection with a NO-specific biosensor.2. We present here the first direct evidence that noradrenaline elicits long-lasting NO production in C6 cells pretreated with lipopolysaccharide and interferon- , an effect blocked by NG-monomethyl-L-arginine, a NO synthase inhibitor.3. This direct electrochemical measurement of glia-derived NO should facilitate our understanding of the kinetics of glial signaling in glia-glia and glia-neuron networks in the brain. |
| Keywords: | nitric oxide noradrenaline C6 glioma cells direct electrochemical monitoring |
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