Direct somatic embryogenesis from axes of mature peanut embryos |
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Authors: | A H McKently |
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Institution: | (1) The Land, EPCOT Center, P.O. Box 10,000, 32830 Lake Buena Vista, Florida |
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Abstract: | Summary Plant regeneration via somatic embryogenesis was obtained in peanut (Arachis hypogaea L.) from axes of mature zygotic embryos. The area of greatest embryogenic activity was a 2-mm region adjacent to and encircling
the epicotyl. Somatic embryogenesis was evaluated on Murashige and Skoog media supplemented with a variety of auxin treatments.
Maximum production occurred on medium supplemented with 3 mg · liter−1 4-amino-3,5,6-trichloropicolinic acid. Explant cultures were transferred to half-strength medium supplemented with 1 mg ·
liter−1 gibberellic acid for somatic embryo germination and early plantlet growth. Plantlets, transferred to soil, were placed in
a greenhouse and grown to maturity. |
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Keywords: | Arachis hypogaea L somatic embryogenesis tissue culture auxin picloram |
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