Effect of exogenous cytokinins on growth and somatic embryogenesis in anise cells (Pimpinella anisum L.) |
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Authors: | Dietrich Ernst Dieter Oesterhelt |
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Institution: | (1) Max-Planck-Institut für Biochemie, D-8033 Martinsried, Federal Republic of Germany |
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Abstract: | A cell culture of anise was grown in the presence or absence of 2,4-dichlorophenoxyacetic acid (2,4-D). Application of isopentenyladenine or isopentenyladenosine (4·10-8 to 4·10-7 M) to the proembryonic culture (+2,4-D) yielded an increase of the cell density, in contrast to a proembryonic culture grown without exogenous application of cytokinins. Embryogenesis was induced by transferring the cells to a hormone-free medium. Embryo development was promoted by isopentenyladenine and isopentenyladenosine (5·10-8 to 5·10-7 M), higher concentrations (5·10-6 M) inhibited embryogenesis. The effect of cytokinins on embryogenesis was only promotive until the third day of culture, i.e. coincident with cell growth rather than differentiation.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- i6Ade
isopentenyladenine
- i6Ado
isopentenyladenosine |
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Keywords: | Cell culture Cell growth Cytokinin and embryogenesis Embryogenesis in cell culture Pimpinella |
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