An enhanced microsatellite map of diploid Fragaria |
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Authors: | D J Sargent J Clarke D W Simpson K R Tobutt P Arús A Monfort S Vilanova B Denoyes-Rothan M Rousseau K M Folta N V Bassil N H Battey |
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Institution: | (1) East Malling Research (EMR), New Road, East Malling, Kent, ME19 6BJ, UK;(2) Departament de Genética Vegetal, Institut de Recerca i Tecnologia Agroalimentáries (IRTA), Carretera de Cabrils s/n, 08348 Cabrils (Barcelona), Spain;(3) INRA-Unité de Recherche sur les Espèces Fruitières et la Vigne, BP 81, 33883 Villenave d’Ornon Cedex, France;(4) Plant Molecular and Cellular Biology Program and Horticultural Sciences Department, The University of Florida, P.O. Box 110690, 1301 Fifield Hall, Gainesville, FL 32611, USA;(5) USDA-ARS NCGR, 33447 Peoria Road, Corvallis, OR 97333, USA;(6) School of Plant Sciences, The University of Reading, Whiteknights, P.O. Box 221, Reading, RG6 6AS, UK |
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Abstract: | A total of 45 microsatellites (SSRs) were developed for mapping in Fragaria. They included 31 newly isolated codominant genomic SSRs from F. nubicola and a further 14 SSRs, derived from an expressed sequence tagged library (EST-SSRs) of the cultivated strawberry, F. × ananassa. These, and an additional 64 previously characterised but unmapped SSRs and EST-SSRs, were scored in the diploid Fragaria interspecific F2 mapping population (FV×FN) derived from a cross between F. vesca 815 and F. nubicola 601. The cosegregation data of these 109 SSRs, and of 73 previously mapped molecular markers, were used to elaborate an enhanced
linkage map. The map is composed of 182 molecular markers (175 microsatellites, six gene specific markers and one sequence-characterised
amplified region) and spans 424 cM over seven linkage groups. The average marker spacing is 2.3 cM/marker and the map now
contains just eight gaps longer than 10 cM. The transferability of the new SSR markers to the cultivated strawberry was demonstrated
using eight cultivars. Because of the transferable nature of these markers, the map produced will provide a useful reference
framework for the development of linkage maps of the cultivated strawberry and for the development of other key resources
for Fragaria such as a physical map. In addition, the map now provides a framework upon which to place transferable markers, such as genes
of known function, for comparative mapping purposes within Rosaceae. |
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Keywords: | Fragaria Genetic mapping Microsatellites EST Functional genomics |
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