Characterization of S-layers from mesophilic bacillaceae and studies on their protective role towards muramidases |
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Authors: | Margit Sára Karin Moser-Thier Ursula Kainz Uwe B Sleytr |
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Institution: | (1) Department of Biochemistry and Biophysics, Chalmers University of Technology, S-412 61 Göteborg, Sweden;(2) University of Göteborg, S-412 61 Göteborg, Sweden;(3) Department of Marine Microbiology, University of Göteborg, Carl Skottsbergsgatan 22, S0423 19 Göteborg, Sweden;(4) Present address: Department of Medical Biochemistry, University of Göteborg, Box 33031, S-400 33 Göteborg, Sweden |
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Abstract: | High resolution 13C NMR combined with chemical analysis were used to study the formation of metabolites from 1-13C]-labelled glucose by the salt-tolerant yeast Debaryomyces hansenii after transfer to media containing 8% NaCl. Time course spectroscopy of an aerobic cell suspension showed 1,3-13C]glycerol as the predominant end product. Perchloric acid extracts revealed additional less prominent incorporation of label into arabinitol, trehalose, glutamic acid, and alanine. The incorporation into trehalose and arabinitol showed a transient increase after shift to the high salinity medium. It is concluded that glycerol and arabinitol are the major organic solutes in D. hansenii, the production of glycerol being strongly induced by high salinity. Analysis of labelled extracts of D. hansenii after transfer to 8% NaCl media containing 1-13C]- or 6-13C]glucose, demonstrated that glucose is dissimilated via a combination of the Embden-Meyerhof-Parnas pathway and the pentose phosphate pathway, with the former playing a major role in glycerol formation and the latter in arabinitol production. The almost exclusive labelling of C5 of arabinitol from 6-13C]glucose indicates that the pathway to arabinitol proceeds via reduction of ribulose-5-phosphate.Abbreviations used NMR
nuclear magnetic resonance
- EMP
Emden-Meyerhof-Parnas
- PP
pentose phosphate
- GAP
glyceraldehyde phosphate
- DHAP
dihydroxyacetone phosphate
- ppm
parts per million |
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Keywords: | 13C NMR spectroscopy Yeast Debaryomyces hansenii Osmoregulation Compatible solute Salt stress |
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