用人/啮齿类体细胞杂种系及荧光原位杂交将人类新基因ZNF191定位于染色体18q12.1

本文以锌指蛋白ZNF191全长cDNA为探针,与人/啮齿类体细胞杂种系DNA杂交,将这个新的人类锌指基因定位于18号染色体。又用该cDNA筛选人基因组DNA lambda/DASH文库,以获得的DNA片段为探针,进行人染色体荧光原位杂交(FISH)分析,将ZNF191基因精细定位在染色体18q 12.1区带。依据有关遗传连锁分析和等位基因荧光原位杂交(FISH),将ZNF191精确定位于人染色体18q12.1。通过遗传连锁及染色体杂合性丢失分析.目前已知多种遗传病和肿瘤与这个区域相关。因此,ZNF191基因可作为这些疾病或肿瘤的候选相关基因。

ASSIGNMENT OF A NOVEL ZINC FINGER GENE ZNF191 TO HUMAN CHROMOSOME 18Q12.1 BY HUMAN/RODENT SOMATIC CELL HYBIRD PANEL AND FLUORESCENT IN SITU HYBRIDIZATION

A novel human zinc finger gene, ZNF191,was assigned to chromosome 18 by hybridization of human/rodent hybrid cell panel to a full-length cD-NA as a probe. Meanwhile,a human genomic DNA lambda/DASH library was screened using this cD-NA probe and several positive clones were obtained. Fluorenscence in situ hybridization (FISH) was performed by using one of these positive clones,16-1,as a probe. Thus,the ZNF191 gene was precisely mapped in 18q12.1. To date, some hereditary diseases and tumors have been found to be associated with this region by analysis of genetic linkage and loss of heterozygosity. Hence, it suggested that the gene ZNF191 can be taken as a candidate gene responsible for those diseases and tumors.