Increased PC-1 phosphodiesterase activity and inhibition of glucose uptake in adipocytes of type 2 diabetic rats |
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| Authors: | Karlene Barrett Donovan McGrowder Paul Brown Dalip Ragoobirsingh |
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| Institution: | (1) Departments of Basic Medical Sciences (Biochemistry Section), University of the West Indies, Mona, Kingston 7, Jamaica;(2) Departments of Pathology, University of the West Indies, Mona, Kingston 7, Jamaica;(3) Department of Basic Medical Sciences (Biochemistry Section), University of the West Indies, Mona Campus, Kingston 7, Jamaica, West Indies |
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| Abstract: | This study was designed to understand the cellular mechanisms responsible for defects in the insulin-stimulated signal transduction pathway in a type 2 diabetic animal model. We examined the in vitro PC-1 phosphodiesterase activity and glucose uptake in adipose tissue of streptozotocin (STZ)-induced type 2 diabetic rats. The PC-1 activity was significantly increased in adipose tissue of diabetic rats (0.54 ± 0.08 nmol PNTP hydrolyzed/mg protein/min) compared with controls (0.29 ± 0.05 nmol PNTP hydrolyzed/mg protein/min, p < 0.05). Upon insulin stimulation (100 nM), glucose uptake in the adipose tissue of the controls (4.17 ± 1.28×10−8 μmol/mg/min) was significantly higher than that in the diabetic rats (1.26 ± 0.35×10−8; p < 0.05). These results suggest that elevated PC-1 phosphodiesterase activity and decreased glucose uptake in adipose tissues may be acquired characteristics contributing to the development of type 2 diabetes mellitus. |
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| Keywords: | adipocytes glucose uptake PC-1 phosphodiesterase activity type 2 diabetes |
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